Investigation of the response of tear-film neutrophils to interleukin 8 and their sensitivity to centrifugation, fixation, and incubation.

While changes in level of expression of CD16 across collection days did not reach statistical significance, large variability in CD16 expression was observed not only across days but within single participant collection data, where for example on day 1, a MFI of 56 would be recorded, 5 on day 2 and then 34 on day 29.

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