Climatic and topographic changes since the Miocene influenced the diversification and biogeography of the tent tortoise (Psammobates tentorius) species complex in Southern Africa.

7 ) and ENM analyses in the LGM (Additional file 1 : Table S9), an interesting trend was observed, namely, that a decrease in suitable range generally correlated with an increase in diversification rates, and vice versa.

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Besides this, our haplotype based diversification rate analysis revealed an increasing trend of diversification that corroborated our ENM results for Pv-A.

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By contrast, Ptt-B and Pv-B showed a remarkable decline in their λ plots, indicating a decreasing trend in net diversification.

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tentorius species complex showed a trend of increasing diversification rates after its cladogenesis started approximately 10 Ma, levelling off only in recent time (approximately 1 Ma, Fig. 7 b).

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