did not reach statistical significanceP = 0.0198
The higher BiP expression in the MU group was also supported by western blotting analysis, although the difference of the BiP/FLAG ratio did not reach statistical significance, probably due to the inclusion of FLAG-negative cells (BiP/FLAG, WT vs MU: P = 0.0198; BiP/Acitn, WT-H 2 O 2 vs MU-H 2 O 2 : P = 0.0102; Figure 4D ).