Altering the pre-aptamer leader sequence unmasks a strong influence of P1 sequence on regulatory activity One possibility for the lack of a clear trend in fold induction within the above set of P1 variants is that sequence changes in P1 result in alternative structure(s) that interfere with formation of functional structures.
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Requirements for efficient ligand-gated co-transcriptional switching in designed variants of the B. subtilis pbuE adenine-responsive riboswitch in E. coli.
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