Identification of genes associated with the biosynthesis of unsaturated fatty acid and oil accumulation in herbaceous peony 'Hangshao' (Paeonia lactiflora 'Hangshao') seeds based on transcriptome analysis.

1 c), it was found that the content of ALA, LA and PA showed a trend toward first rising and then falling during seed development, and the maximum value appeared in the S4 period.

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Although most of the FPKM values of SAD , FAD2 and FAD3 based on the transcriptome sequencing showed a decreasing trend with seed development, their expression levels were highest at 45 DAF by qRT-PCR analysis and exhibited a bell shape with seed development (Fig. 8 ), consistent with the rate of unsaturated fatty acid accumulation (Fig. 1 c).

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