A clear trend following a very similar pattern of monolayer damage was observed when E. histolytica trophozoites treated with ACh supernatants were added to HepG2 cell monolayers, demonstrated that 0.0001 μM ACh stimulation promoted contact-independent cell killing ( Figure 7C ).
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Acetylcholine Upregulates <i>Entamoeba histolytica</i> Virulence Factors, Enhancing Parasite Pathogenicity in Experimental Liver Amebiasis.
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