Thus, our qPCR analysis demonstrated that the CB 1 receptor was downregulated in the cerebellum, with a small trend toward a decrease in the hippocampus ( p = 0.179) of Syn-Cre/Scn1a WT/A1783V mice ( Figure 10A ), which was corroborated by western blot analysis ( Figure 11E ).
In parallel, we also measured gene expression of the proinflammatory cytokine TNF-α, which was apparently elevated in different CNS structures of Syn-Cre/Scn1a WT/A1783V mice, in particular in the hippocampus ( p = 0.065), although these differences did not reach statistical significance in any case, likely due to a high variation in these mice ( Figure 8A ).