Flow cytometric analysis on day 7 demonstrated that V-iTregs as well as C-iTregs showed a trend toward suppressing allograft infiltration of CD45 + leukocytes, CD4 + T cells, CD8 + T cells, and F4/80 + macrophages compared with that of the control group without iTreg transfer, albeit without statistical significance ( Fig 4C ).
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Suppressive effects of vitamin C-treated induced-regulatory T cells on heart allograft rejection under vitamin C-deficient or -sufficient conditions.
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