Quantification of Cas9 binding and cleavage across diverse guide sequences maps landscapes of target engagement.

Sci Adv · 2021 · PMC7895440 · PMID 33608277

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an important trendno p-value reported

The off-target cleavage data revealed an important trend: Most targets with 15 or 16 bp of complementarity exhibited an intermediate level of final cleavage.

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