Effects of storage time and temperature on highly multiparametric flow analysis of peripheral blood samples; implications for clinical trial samples.

After processing, a much greater loss of WBCs was seen for refrigeration times of 6 h (62%) and 24 h (74%), while a small increase for samples stored at RT did not reach statistical significance ( Figure 2 A).

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Examination of whether the extent of neutrophil contamination was related to down-regulation of the chemokine receptors CXCR5 and CCR6, which showed the largest effect of RT storage, indicated a highly significant correlation.

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