This correlated with the Western blotting data, which showed a trend of decreasing H3K9 as the pillar size/spacing increased, leading from a threefold reduction in total H3K9 in cells on the 1 × 5 micropillars to a fivefold decrease in cells on both the 5 × 5 and 10 × 5 designs (Figure 4g and Figure S8g, Supporting Information).
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Precision Surface Microtopography Regulates Cell Fate via Changes to Actomyosin Contractility and Nuclear Architecture.
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