At a test dose of 0.5 μg/mL, we found that all three predicted peptides stimulated a highly significant increase in GLUT4 translocation ( Figure 2 B).
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Discovery through Machine Learning and Preclinical Validation of Novel Anti-Diabetic Peptides.
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The pentose phosphate pathway, an alternative pathway to glycolysis, and the citrate cycle for oxidation of glucose also showed a trend toward enrichment when treated with pep_1E99R5, as did the TGF-β pathway, shown to stimulate the glucose uptake through GLUT1 [ 61 ].