Barely Significant
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SARS-CoV-2 outbreak in a tri-national urban area is dominated by a B.1 lineage variant linked to a mass gathering event.

PLoS Pathog · 2021 · PMC8011817 · PMID 33740028

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a negative trendno p-value reported
In general, we observed a negative trend linking C t values and percentage of ambiguous bases (Ns) being called as a result of low coverage.

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Quoted from the open-access full text in Europe PMC under the licence the publisher applied. The sentence is reproduced exactly as published; the emphasis is ours.