WB analysis of renal OM tissue (where mTALs are the most abundant tubules) showed a trend for mTORC2 activation in H 2 O 2 infused rats compared to saline infused rats, however it did not reach significance ( p = 0.11) (Fig. 1 C).
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Hydrogen peroxide (H<sub>2</sub>O<sub>2</sub>) mediated activation of mTORC2 increases intracellular Na<sup>+</sup> concentration in the renal medullary thick ascending limb of Henle.
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