Importantly, the number of live/dead cells generated over both assays was different (Figure 2a ; top corner ANOVA, n = 15, p = 0.0001) showing highly significant difference at day 6 with a mean 8‐fold more live cells generated in TD but only 1.6‐fold in TI ( n = 15, p = 0.0001).
We also compared male ( n = 7) and females ( n = 8) and observed no significant differences in circulating subset frequency, marker levels of expression, % of + cells, Ig production and gene expression between gender groups (Figures S3 , S4 and S7 ), while cell counts showed a trend for higher live cell means during female B‐cell expansion in both TD and TI assays at day 6, as well as for the generation of live PC at day 13 (not significant). 3.2.