Expression and Roles of Individual HIF Prolyl 4-Hydroxylase Isoenzymes in the Regulation of the Hypoxia Response Pathway along the Murine Gastrointestinal Epithelium.

Caecum Epithelium In the caecum epithelium Hif1a expression reached 242 ± 97 copies/fg RNA input at 8 weeks of age and thus exceeded that of Hif2a (171 ± 88 copies/fg RNA input), although this difference did not quite reach statistical significance ( Figure 1 B).

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Hif-p4h-2 mRNA had the highest abundance (44 ± 15 copies/fg RNA input) among the Hif-p4h isoforms, which was significantly higher than that of Hif-p4h-1 (18 ± 7 copies/fg RNA input) and Fih1 (15 ± 8 copies/fg RNA input), while the difference between the Hif-p4h-2 and Hif-p4h-3 (30 ± 15 copies/fg RNA input) mRNA levels did not reach statistical significance ( Figure 1 C).

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