Digital PCR Detection of mtDNA/gDNA Ratio in Embryo Culture Medium for Prediction of Embryo Development Potential.

20 The mtDNA/gDNA ratio increased as that of embryo fragments increased, but the difference did not reach statistical significance.

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With the multiple annealing and looping-based amplification cycles method of next-generation sequencing for whole genome amplification, a study reported that the mt/gDNA ratio of medium from embryos that reached blastulation with successful pregnancy showed a decreasing trend, but the differences were not statistically significant. 23 Based on previous research, we reduced the impact of individual differences and the influence of age by selecting standard patients in our center seeking assistance with pregnancy.

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