miR-200c-3p Regulates Epitelial-to-Mesenchymal Transition in Epicardial Mesothelial Cells by Targeting Epicardial Follistatin-Related Protein 1.

By qRT-PCR, we verified the up-regulation of miR-200c main targets, i.e., ZEB1 and ZEB2 ( Figure S3A,B ) after 48, 72, and 96 h of TGF β1 treatment and at all doses: the up-regulation of ZEB1 was significant, mainly after 48 h of TGF β1 stimulation, while the modulation of ZEB2 showed a trend that was never significant. qRT-PCR results were confirmed by WB analysis, showing a strong enhancement in ZEB1 protein expression, but at a later time point (72 h instead of 48 h) ( Figure S3C ). 2.3.

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