Establishment of open-source semi-automated behavioral analysis system and quantification of the difference of sexual motivation between laboratory and wild strains.

It was found that the latency to first following approached significance, suggesting a difference (Kiyosu; 2.763 ± 0.602, d-rR; 0.963 ± 0.540, P = 0.070 (Fig. 6 B)).

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The latency to first following was shorter almost significantly (Fig. 6 b) and the frequency of courtship before spawning were significantly higher in d-rR (Fig. 6 e).

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