moderately significantp < 0.001
Statistical analysis A statistical analysis of collected data was carried out to highlight the significant differences between the yields of the three kinds of extraction used and investigated species, through the use of GraphPad prism 8.2.263 software, by two-way analysis of variance (ANOVA), then Tukey test to detect the degree of significance, this significance is taken at the probability of ∗p < 0.05 for a significant difference, ∗∗p < 0.01 for moderately significant difference, ∗∗∗p < 0.001 for a highly significant difference, ∗∗∗∗p < 0.0001 for a very highly significant difference (a high value of F means that something is significant, while a small value of p means that all results are significant, the null hypothesis (H0) is rejected from p < 0.05). 2.5.