Evaluation of protein secondary structure from FTIR spectra improved after partial deuteration.

Eur Biophys J · 2021 · PMC8189984 · PMID 33534058

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Regarding the comparison between FTIR data obtained for protein solution and “dry” film, it must be stressed that, as discussed above, the amount of water left in “dry” proteins is quite significant and the validity of “dry” film has been reviewed elsewhere (Goormaghtigh et al. 1999 ).

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