Selection of established tumour cells through narrow diameter micropores enriches for elevated Ras/Raf/MEK/ERK MAPK signalling and enhanced tumour growth.

Flow-sorting resulted in a similar shift from irregular to elongated morphologies, although this change did not reach statistical significance.

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In fact, there was even a slight trend towards reduced proliferation of the Pore-Selected and Flow-Sorted cells relative to Parent cells, albeit insufficient to achieve statistical significance.

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