In these comparisons, although the differences in K d values were apparent, they did not reach statistical significance (Nluc-FZD 4 K d (nM) ± SEM = 7.6 ± 3.6; HiBiT-FZD 4 setup 1 K d (nM) ± SEM = 11.9 ± 3.6; HiBiT-FZD 4 setup 2 K d ± SEM (nM) = 20.4 ± 7.6).
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Quantitative Profiling of WNT-3A Binding to All Human Frizzled Paralogues in HEK293 Cells by NanoBiT/BRET Assessments.
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