Thin-Layer-Agar-Based Direct Phenotypic Drug Susceptibility Testing on Sputum in Eswatini Rapidly Detects Mycobacterium tuberculosis Growth and Rifampicin Resistance Otherwise Missed by WHO-Endorsed Diagnostic Tests.

When inoculation was performed within 4 days, or from 5 days or later from sample collection, the TLA positivity rate for Sm + samples decreased from 72.6% to 59.0% ( P = 0.053), while the negative rate increased from 20.1% to 28.3.8% ( P = 0.16), though it did not reach statistical significance.

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