Vaccinia virus hijacks ESCRT-mediated multivesicular body formation for virus egress.

In line with the EEV yields, depletion of CHMP4B and CHMP5 showed a trend of increased colocalization, whereas knockdown of CHMP1A, CHMP3, CHMP4C, or CHMP6 resulted in 47%, 70%, 27%, and 72% less colocalization, respectively, when compared with control cells ( Fig 4B ).

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