Reference Gene Selection for Gene Expression Analyses in Mouse Models of Acute Lung Injury.

However, while the inter-group comparison between pH 2.0 and the two other treatment groups was significantly different if data were normalised to the optimised RGI ( Figure 3 D II , green: Rpl13a/Rps29 ), these comparisons did not reach statistical significance in the case of unsuitable normalisation ( Figure 3 D I , red: Actb/Ywhaz ).

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