Development of a Bispecific Antibody-Based Platform for Retargeting of Capsid Modified AAV Vectors.

However, high amounts of bispecific antibodies binding viral capsids showed a trend to reduced transduction frequencies, which was possibly due to generation of large antibody–rAAV-2E3 complexes which may have inhibited internalization or promoted degradation by the proteasome after internalization. rAAV-2E3.v6 on its own did not induce GFP expression in cells, as expected, but increasing amounts of GFP positive cells and consequently increasing MFIs were directly linked with the ratio of KiH-2E3-MO33 or KiH-2E3-MO36 molecules per vial genome ( Figure 5 A,F–H).

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