Other osteogenic marker genes (ALPL, alkaline phosphatase, Figure 5 B; SPP1, osteopontin, Figure 5 C; and BGLAP, osteocalcin, Figure 5 D) showed a trend towards induction after stimulation with Sema3A beyond the effect of the osteogenic medium (mineralization medium: min. med. = DMEM, supplemented with 50 mg/mL ascorbic acid, 10 mM ß-glycerophosphate, 10-7 M dexamethasone).
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