Video bioinformatics analysis of human pluripotent stem cell morphology, quality, and cellular dynamics.

Values were not significantly different at any time, although they often came close to significance.

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While most dead cells appear to remain attached to the colonies and there are clearly significant differences between colonies on different matrices, we cannot exclude the possibility that some dead cells detached from colonies leading to an underestimation of cell death. 3.5 Effect of culture media on morphology, growth, death, and motility To determine how colony behavior was affected by different culture media, H9 cells were plated on Matrigel and grown in mTeSR1‐A or mTeSR1‐B (batches purchased at different times), E8, and mTeSR2.

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