Deep and accurate detection of m6A RNA modifications using miCLIP2 and m6Aboost machine learning.

Generally, introns harboring m 6 A modifications showed a significant trend towards more IR reduction compared to unmodified introns (Figure 4I ), indicating that modifications on retained introns may directly influence splicing efficiency. m6Aboost can be applied to predict m 6 A sites in human cells To test m6Aboost on miCLIP2 data from a different species, we performed miCLIP2 experiments with poly(A)+ RNA from human HEK293T cells ( n = 4 replicates with 30 million truncation events on average, Supplementary Figure S1F and G ).

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The latter were enriched in lowly expressed genes and most likely failed to reach significance in the differential methylation analysis due to low read counts ( Supplementary Figure S4F ).

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