Optimization and Validation of a Novel Three-Dimensional Co-Culture System in Decellularized Human Liver Scaffold for the Study of Liver Fibrosis and Cancer.

HepG2 cells grown in mono-culture and SimCC showed a similar tendency towards increased EPCAM protein expression while those in SeqCC showed a trend towards downregulation of EPCAM expression compared to SimCC ( Figure 2 A), confirming the FACS analysis data that demonstrated more LX2 cells than HepG2 cells in SeqCC ( Figure 1 A).

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