Structure-based charge calculations for predicting isoelectric point, viscosity, clearance, and profiling antibody therapeutics.

It has generally been observed that removing negatively charged residues or adding positively charged residues to the CDRs can reduce viscosity for IgG1 antibodies and this most recent dataset is consistent with that observation, as a clear trend is seen where the higher the Fv charge, the lower the viscosity. 9–13 The methods developed for computational viscosity prediction in these studies generally demonstrate this same premise.

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