RNA sequencing reveals metabolic and regulatory changes leading to more robust fermentation performance during short-term adaptation of Saccharomyces cerevisiae to lignocellulosic inhibitors.

Apart from a protein of unknown function (DPA10), three out of four genes that showed a trend of down-regulation (Fig. 6 C) encoded for transporters.

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DHA1 multidrug family antiporters Yhk8 and Flr1 YHK8 and FLR1 , both members of the multidrug proton antiporter DHA1 family [ 70 , 71 ], showed significant differential expression from t2 (21.5 h) onwards, together with an increasing trend, reaching high logFC values (Fig. 6 A).

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