A Promiscuous Bacterial P450: The Unparalleled Diversity of BM3 in Pharmaceutical Metabolism.

In the above examples of promiscuous variants, there is a clear trend of the introduction of the same or similar point mutations which expand BM3′s catalytic repertoire, including: the neutralization of the polar binding mouth of the active site granting access to non-polar substrates (R47L/Y51F), the addition of polar residues to counteract an overly non-polar environment (L188Q), the removal of Phe87 steric hindrance (F87A/V/L/G) and active site reorganizations (A82L/F, A74E, V78L/F).

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