We found that alike in RPE1 cells, the number of cells expressing UFBs was significantly increased in DMSO treated H3.3K27M compared to H3.3WT U251 cells, and there was a clear trend towards an increase in APH treated H3.3K27M cells ( Fig 4E ).
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The H3.3K27M oncohistone affects replication stress outcome and provokes genomic instability in pediatric glioma.
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