Recombinase Polymerase Amplification/Cas12a-Based Identification of <i>Xanthomonas arboricola</i> pv. <i>pruni</i> on Peach.

For the optimization of Cas12a concentration, the fluorescence signal intensity showed a trend of increasing first and then decreasing from 50 to 350 nM, reaching a peak at 150 nM ( Figure 2B ).

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