Depletion of METTL3 alters cellular and extracellular levels of miRNAs containing m⁶A consensus sequences.

For ALKBH5, miR-100 levels trended up in cells and down in EVs, but did not reach statistical significance in either case. 2.4 Small RNA profiles after knockdown of METTL3 and ALKBH5 are distinct between cells and EVs To more quantitatively and comprehensively assess the effects of depletion of METTL3 and ALKBH5 on cellular and EV RNA profiles, we conducted RNAseq on libraries created from small RNAs purified from control parental DKO-1 cells and EVs, and from knockdown cells and EVs (Figure S4; Table S2).

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