Light sets the brain's daily clock by regional quickening and slowing of the molecular clockworks at dawn and dusk.

Additionally, as entrainment to 8:16 skeleton photoperiods causes period lengthening of circadian behavior in vivo ( Pittendrigh and Daan, 1976b ), it induced a trend toward period lengthening of PER2::LUC rhythms as an after-effect (0.47 ± 0.11 hr), although these did not reach statistical significance ( Figure 4E , Figure 4—figure supplement 2 ).

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