Using Unnatural Protein Fusions to Engineer a Coenzyme Self-Sufficiency System for D-Phenyllactic Acid Biosynthesis in <i>Escherichia coli</i>.

Front Bioeng Biotechnol · 2021 · PMC8718758 · PMID 34976983

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showed a trendno p-value reported

D-PLA titer increased with the increase of PPA concentration, but the yield showed a trend of continuous decline, because the increase of PPA concentration enhanced the toxic effect on E. coli CP303.

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an increasing trendno p-value reported

However, the intracellular NADH concentration of co-expressing strains (CP201, CP202, CP203, and CP204) with PPA and glycerol as co-substrates showed an increasing trend.

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