Conformational decoupling in acid-sensing ion channels uncovers mechanism and stoichiometry of PcTx1-mediated inhibition.

A striking difference was apparent in the washout: unlike in K105C*, the fluorescence signal of K105C*F350L readily returned to a level indicative of a closed apo state in the first 1–2 min of pH 7.4 washout, suggesting that the longevity of the ‘ECD only ’ state is destabilized ( Figure 3G , Supplementary file 1d ).

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