Barely Significant
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Comparison of analytical sensitivity and efficiency for SARS-CoV-2 primer sets by TaqMan-based and SYBR Green-based RT-qPCR.

Appl Microbiol Biotechnol · 2022 · PMC8881549 · PMID 35218386

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slightly significantno p-value reported
However, if this could be corrected, the bespoke reverse primer would only have one slightly significant mismatch (C-T) for Lambda variants.

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