near-significantP = 0.06
The average levels of reads mapped to a locus expressed in MCF7 (71.2 reads, n = 162 loci) was significantly higher than in HeLa (29.8 reads, P = 0.005, Student's t -test) and near-significant in HEK293 (41.4 reads, P = 0.06, Student's t -test), although the HeLa results are partially a reflection of the lower threshold of mapped reads for expression (see Methods).