a significant trendp = 0.058
Due to much larger variability of the mapped pain areas compared to NRS pain rating, ANOVA of the pain distributions revealed only a significant trend effect for “tissue” (F = 4.2, p = 0.058), but a significant main effect of “volume of injection” (F = 5.3, p < 0.01), while there was no interaction at all between both (F = 0.1, p = 0.926; two way RM-ANOVA, Figure 5 A).