Process optimization for the synthesis of functionalized Au@AgNPs for specific detection of Hg²⁺ based on quality by design (QbD).

The results showed that X 1 had an extremely significant effect on the performance of Au@AgNPs with P -value < 0.0001, X 2 and X 4 reached a significant level with P -value < 0.05, while X 3 , X 5 and X 6 had no significant effects on the performance of Au@AgNPs.

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6A , the Raman signal showed a trend of enhancing and then decreasing with increasing Dpy concentration.

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The regression model had a p -value < 0.0001, indicating that the model was highly significant.

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