approached significancep = 0.059
Quantification of the acetylated tubulin again showed that both the 3D system (HD or LM) and time of IVC (24 or 48 h) significantly affected the levels of acetylated tubulin in the entire set of samples ( p < 0.001 and p = 0.001, respectively) while the cell concentration approached significance ( p = 0.059).