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Detailed Analysis of Zebrafish Larval Behaviour in the Light Dark Challenge Assay Shows That Diel Hatching Time Determines Individual Variation.

Front Physiol · 2022 · PMC9036179 · PMID 35480044

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a clear trendno p-value reported
Moreover, when separating locomotion patterns of individuals hatched during daytime from those of individuals hatched during nighttime, the results showed a clear trend: day hatched individuals had higher values of movement related parameters D and V, and lower values of directionality M and Ω, during the dark phase of the behavioural test than night hatched individuals.

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