In line with this, our MS analysis showed a trend that both K27‐ and K29‐linked Ub modifications accumulated on Ub(G76V)‐GFP upon inhibition of p97, although in the case of K27 linkages, the increase was modest and varied between individual samples (Fig 4F ).
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K27-linked ubiquitylation promotes p97 substrate processing and is essential for cell proliferation.
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