did not reach statistical significancep = 0.0497
The RT-PCR results showed that the expression patterns of the core clock genes Bmal1 , Per1 , and Per2 and the clock-controlled genes Avp and Pk2 showed slightly different patterns but did not reach statistical significance except for Bmal1 (p = 0.0497) in the ZT0-4 TRF group ( Figure S8 ).