Metabolic Fingerprint Analysis of Cytochrome <i>b</i> ₅-producing <i>E. coli</i> N4830-1 Using FT-IR Spectroscopy.

The PC–DFA scores plots also displayed a clear trend for the induced samples according to the gene copy number from N0–N6, which was confirmed and illustrated in the findings of all the three separate batch cultures, highlighting the reproducibility of the whole process ( Supplementary Figure S6 ).

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Using the established optimum concentration of hemin (60 μM), an increasing trend of Cyt b 5 content was detected and correlated directly with increasing gene copy numbers, as shown in Figure 5C .

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