Human Cholangiocytes Form a Polarized and Functional Bile Duct on Hollow Fiber Membranes.

Interestingly, the cholangiocyte markers HNF1β, KRT7 , ABCB1 , SLC10A2 , CFTR , and AQP1 showed a trend toward upregulation in CLCs cultured on HFMs as compared to plates cultured under differentiating conditions, suggesting that some cholangiocyte markers show a more mature phenotype in these tubular structures ( Figure 2 ).

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